Molecular biology and bioinformatics

Molecular biology and bioinformatics

  • Analysis and optimisation of gene expression
  • Comprehensive transcriptome analysis of cells based on high-density microarrays with bioinformatics analysis of results
  • Analysis of selected gene expression, miRNA with qRT-PC
  • Real-time monitoring of gene expression, RT-PCR
  • DNA, RNA, miRNA and protein isolation from various types of biological materials
  • DNA analysis and modification
  • PCR analysis with primer construction
  • NA, RNA and protein quality estimation with bioanalyser
  • DNA Methylation analysis with High Resolution Melt (HRM)
  • Microbial identification (traditional and molecular methods)
  • Molecular cloning and bacterial expression vector design
  • Building up and screening of mutants libraries
  • In agarose and polyacrylamide gel (separation of proteins and nucleic acids)
  • Two-dimensional electrophoresis (2-DE) of proteins
  • Separation of proteins and nucleic acids with on-chip electrophoresis
  • Two-dimensional difference gel electrophoresis (2D DIGE)
  • High-resolution DNA electrophoresis within 50–700 bp (resolution 1–4 nt)
  • Capillary electrophoresis
  • Site-directed mutagenesis of proteins
  • Quantitative fluorymetric analysis of proteins and nucleic acids
  • Recombinant protein expression and optimisation (RT-PCR, Western Blot)
  • Western Blotting, ELISA test
  • Immunodetection/chemiluminescence of proteins
  • Identification of proteins digested with a proteolytic enzyme (MALDI-TOF/TOF, MALDI‑TOF/TOF +nanoHPLC)
  • Analysis of protein lysates (Shotgun Proteomics analysis)
  • Measurement of the mass of a protein, peptide or enzymatically digested protein (MALDI‑TOF/TOF or ESI-MS/MS method)
  • Small genome sequencing (bacteria, yeasts, BAC plasmids etc.)
  • Large genomes sequencing (human, animals, plants)
  • Whole egzome sequencing
  • Small RNA sequencing (microRNA profile)
  • RNAseq (gene expression profile and whole transcriptome sequencing)
  • Transcriptome targeted sequencing
  • Targeted resequencing
  • DNA Methylation/ WGBS – Whole Genome Bisulfite Sequencing
  • SNP and structural variant discovery with NGS method
  • By sequencing (RAD-seq)
  • SNPs genotyping with MALDI-TOF mass spectrometry and real-time PCR
  • Optimization of spatial structure of proteins, lipids and carbohydrates
  • Study on the influance of solvent, ions, radicals and external electromagnetic field
  • On the shape of molecules
  • Study of the  intermolecular interaction energy and dynamics of systems
  • Determination of the influence of functional groups and ligands on the structure and
  • Properties of the molecule
  • Study of molecules conformational changes
  • Metagenomics and microbiome research (16s/ITS or WGS analysis)

Contact

Agata Dominiak

+48 500 032 642

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