Molecular biology and bioinformatics
- Analysis and optimisation of gene expression
- Comprehensive transcriptome analysis of cells based on high-density microarrays with bioinformatics analysis of results
- Analysis of selected gene expression, miRNA with qRT-PC
- Real-time monitoring of gene expression, RT-PCR
- DNA, RNA, miRNA and protein isolation from various types of biological materials
- DNA analysis and modification
- PCR analysis with primer construction
- NA, RNA and protein quality estimation with bioanalyser
- DNA Methylation analysis with High Resolution Melt (HRM)
- Microbial identification (traditional and molecular methods)
- Molecular cloning and bacterial expression vector design
- Building up and screening of mutants libraries
- In agarose and polyacrylamide gel (separation of proteins and nucleic acids)
- Two-dimensional electrophoresis (2-DE) of proteins
- Separation of proteins and nucleic acids with on-chip electrophoresis
- Two-dimensional difference gel electrophoresis (2D DIGE)
- High-resolution DNA electrophoresis within 50–700 bp (resolution 1–4 nt)
- Capillary electrophoresis
- Site-directed mutagenesis of proteins
- Quantitative fluorymetric analysis of proteins and nucleic acids
- Recombinant protein expression and optimisation (RT-PCR, Western Blot)
- Western Blotting, ELISA test
- Immunodetection/chemiluminescence of proteins
- Identification of proteins digested with a proteolytic enzyme (MALDI-TOF/TOF, MALDI‑TOF/TOF +nanoHPLC)
- Analysis of protein lysates (Shotgun Proteomics analysis)
- Measurement of the mass of a protein, peptide or enzymatically digested protein (MALDI‑TOF/TOF or ESI-MS/MS method)
- Small genome sequencing (bacteria, yeasts, BAC plasmids etc.)
- Large genomes sequencing (human, animals, plants)
- Whole egzome sequencing
- Small RNA sequencing (microRNA profile)
- RNAseq (gene expression profile and whole transcriptome sequencing)
- Transcriptome targeted sequencing
- Targeted resequencing
- DNA Methylation/ WGBS – Whole Genome Bisulfite Sequencing
- SNP and structural variant discovery with NGS method
- By sequencing (RAD-seq)
- SNPs genotyping with MALDI-TOF mass spectrometry and real-time PCR
- Optimization of spatial structure of proteins, lipids and carbohydrates
- Study on the influance of solvent, ions, radicals and external electromagnetic field
- On the shape of molecules
- Study of the intermolecular interaction energy and dynamics of systems
- Determination of the influence of functional groups and ligands on the structure and
- Properties of the molecule
- Study of molecules conformational changes
- Metagenomics and microbiome research (16s/ITS or WGS analysis)
